Diagnosis and Risk Factors of Prediabetes and Diabetes in People Living with HIV- Evaluation of Clinical and Microbiome Parameters.

Diabetes is more common among people living with HIV (PLWH), as compared with healthy individuals. In a prospective multicenter study (N = 248), we identified normoglycemic (48.7%), prediabetic (44.4%) and diabetic (6.9%) PLWH. HbA1c and fasting blood glucose (FBG) sensitivity in defining dysglycemia was 96.8%, while addition of oral glucose tolerance test led to reclassification of only 4 patients. Inclusion of 93 additional PLWH with known DM enabled identification of multiple independent predictors of dysglycemia or diabetes: older age, higher BMI, Ethiopian origin, HIV duration, lower integrase inhibitor exposure and advanced disease at diagnosis. Shotgun metagenomic microbiome analysis revealed 4 species that were significantly expanded with hyperglycemia/hyperinsulinemia, and 2 species that were differentially more prevalent in prediabetic/diabetic PLWH. Collectively, we uncover multiple potential host and microbiome predictors of altered glycemic status in PLWH, while demonstrating that FBG and HbA1C likely suffice for diabetes screening. These potential diabetic predictors merit future prospective validation.

Phenomenological assessment of psychedelics induced experiences: Translation and validation of the German Challenging Experience Questionnaire (CEQ) and Ego-Dissolution Inventory (EDI).

Several measures have been designed to assess subjective experiences induced by psychedelic substances or other mind-altering drugs as well as non-pharmacological methods. Recently, two self-report questionnaires have been introduced to measure acute adverse effects following psilocybin ingestion and the phenomenon of ego-dissolution associated with the use of psychedelics, respectively. The 26-item Challenging Experience Questionnaire assesses multiple facets of psilocybin induced experiences on seven subscales, whereas the 8-item Ego-Dissolution Inventory consists of a unidimensional scale. In the present study, these questionnaires were translated into German and their psychometric properties then evaluated in an online survey on psychedelics induced experiences. Confirmatory factor analysis suggested an acceptable fit of the 7-factor structure of the German Challenging Experience Questionnaire with overall good internal consistency for all subscales. The factor structure did not differ based on gender or prior struggle with a psychiatric disorder, furthering the evidence of internal validity. Correlations with the State-Trait-Anxiety Inventory and the Altered States of Consciousness Rating Scale demonstrated convergent validity. Confirmatory factor analysis did not support the hypothesized single-factor structure of the German Ego-Dissolution Inventory and exploratory factor analysis suggested an alternative factor structure, where only five items loaded onto a common factor that was interpreted as ego-dissolution. Internal consistency of this 5-item measure was high and correlation with selected items of the Mystical Experience Questionnaire and Altered States of Consciousness Rating Scale supported convergent validity. Translation and validation of these questionnaires contribute to the advancement of common standards in the psychological and neuroscientific study of altered states of consciousness.